Number of studies
5 New studies
Number of samples
101 New samples
Number of fluids
microRNA entries
2871 mature sequences
2017 hairpin sequences

Number of samples publicly available in SRA

Recent Study Uploads

  • Diversity and Signature of Small RNA in Different Bodily Fluids using Next Generation Sequencing

    Small RNAs are critical components in regulating various cellular pathways. These molecules, which could be tissue-associated or circulating in bodily fluids, have been shown to associate with different tumors. Next generation sequencing on small RNAs is a powerful tool for profiling and discovery, particularly for microRNA (miRNA).In this study, we isolated total RNA from various bodily fluids (blood, leukocytes, serum, plasma, saliva, cell-free saliva, urine and cell-free urine). Then, Illumina's next generation sequencing was used to investigate the distribution and signature of small RNAs in these fluids through intensive bioinformatics analysis performed on the sequencing data.

  • Large differences in small RNA composition between human biofluids - 10 biofluids

    One goal of Phase 1 of our project is to produce reference profiles of miRNAs and other small RNAs in a large and diverse set of biofluids using a systemic approach. This study contains samples associated with 10 different biofluids, and all data (sequencing data, full alignments, etc.) is openly available. Overall design: 10-12 donors each from 12 biofluids using modified TruSeq protocol with randomized adapters

  • Circulating miRNAs. isomiRs and small RNA clusters in human plasma and breast milk

    Circulating small RNAs. including miRNAs but also isomiRs and other RNA species. have the potential to be used as non-invasive biomarkers for communicable and non-communicable diseases. This study aims to characterize miRNAs. isomiRs and small RNA clusters in biofluids. to compare their profiles. and to help in the establishment of standard guidelines. For this purpose. RNA from plasma and breast milk samples from 15 healthy postpartum mothers was extracted. Small RNA libraries were prepared with the NEBNext® small RNA library preparation kit and sequenced in an Illumina HiSeq2000 platform. After an initial quality control. miRNAs. isomiRs and clusters of small RNAs were annotated using seqBuster/seqCluster framework. The average amount of extracted RNA was 81 ng/mL [standard deviation (SD): 41] and 3985 ng/mL (SD: 3767) for plasma and breast milk. respectively. Mean number of good quality reads was 4.04 million (M) (40.01% of the reads) in plasma and 12.5M (89.6%) in breast milk. One thousand one hundred eighty two miRNAs. 74.317 isomiRs and 1.053 small RNA clusters that included piwi-interfering RNAs (piRNAs). tRNAs. small nucleolar RNAs (snoRNA) and small nuclear RNAs (snRNAs) were detected. Samples grouped by biofluid. with 308 miRNAs. 4.737 isomiRs and 778 small RNA clusters differentially detected. In summary. plasma and milk showed a completely different small RNA profile. In both. miRNAs. piRNAs. tRNAs. snRNAs. and snoRNAs were identified. confirming the presence of non-miRNA species in biofluids Overall design: Peripheral blood was collected at 0-48 h post-partum for 15 participants. only 5 samples of plasma pass QC; and breast milk was collected at 48-72 h post-partum for 10 of the participants