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Diversity and Signature of Small RNA in Different Bodily Fluids using Next Generation Sequencing
- miRNA Profiles
- sRNA types distribution
- Differential Expression
- Species distribution
Small RNAs are critical components in regulating various cellular pathways. These molecules, which could be tissue-associated or circulating in bodily fluids, have been shown to associate with different tumors. Next generation sequencing on small RNAs is a powerful tool for profiling and discovery, particularly for microRNA (miRNA).In this study, we isolated total RNA from various bodily fluids (blood, leukocytes, serum, plasma, saliva, cell-free saliva, urine and cell-free urine). Then, Illumina's next generation sequencing was used to investigate the distribution and signature of small RNAs in these fluids through intensive bioinformatics analysis performed on the sequencing data.
List of SRA Experiments