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                    adapter: TGGAATTCTCGGGTGCCAAGG
input: /opt/sRNAtoolbox/sRNAtoolboxweb/upload/UBNMV10I6X5S169/IK_exosomes.fastq.gz
minRC: 2
solid: false
dbPath: /opt/sRNAtoolboxDB
noMM: 2
bedGraph: true
adapterMM: 1
writeGenomeDist: true
mBowtie: 10
output: /opt/sRNAtoolbox/sRNAtoolboxweb/upload/UBNMV10I6X5S169
seed: 20
minReadLength: 15
plotMiR: true
kingdom: animal
guessAdapter: false
removeBarcode: 0
microRNA: hsa
recursiveAdapterTrimming: false
adapterMinLength: 10
species: GRCh38_p10_mp
alignType: n
isoMiR: true
tRNA: GRCh38_p10_genomic_tRNA.fa
maintainOrig: true
                        


Preprocessing results

Quality filtered:
328258 (5.29%)
Adapter trimmed
6114811 (98.58%)
Reads in analysis:
4328932 (69.79%)
Length filtered (min):
1457621 (23.5%)
Raw reads:
6203008

Read Length Distribution of Raw Input Reads

Heat Map

Read Length Distribution of Reads in Analysis

Heat Map

Genome/Library mapping results

Genome mapped reads:
3762644(60.66%)

Graphical Summary

MiR profiling results

Reads mapped to miRNAs:
633248 (18.13%)
Reads mapped to miRBase hairpins:
327 (0.01%)
Detected mature miR:
523 (19.69%)
Detected hairpin miR:
369 (19.25%)
MicroRNA details:
isomiR details:
Genomic tRNA database
Mapped reads in sense direction:
222657(6.37%)
multiple assignment (MA)
single assignment (SA)
Mapped reads in antisense direction:
575(0.02%)
MA antisense
SA antisense
yRNAs
Mapped reads in sense direction:
1473245(42.17%)
multiple assignment (MA)
single assignment (SA)
Mapped reads in antisense direction:
2(0.0%)
MA antisense
SA antisense
vault RNAs
Mapped reads in sense direction:
7664(0.22%)
multiple assignment (MA)
single assignment (SA)
Mapped reads in antisense direction:
4(0.0%)
MA antisense
SA antisense
Ensembl release 91 (ncRNA)
Mapped reads in sense direction:
309345(8.85%)
multiple assignment (MA)
single assignment (SA)
Mapped reads in antisense direction:
142614(4.08%)
MA antisense
SA antisense
RNAcentral database 7.0
Mapped reads in sense direction:
470450(13.47%)
multiple assignment (MA)
single assignment (SA)
Mapped reads in antisense direction:
7013(0.2%)
MA antisense
SA antisense
Ensembl release 91 (cDNA)
Mapped reads in sense direction:
106419(3.05%)
multiple assignment (MA)
single assignment (SA)
Mapped reads in antisense direction:
120009(3.44%)
MA antisense
SA antisense
tRNA anticodon statistic:
details